STUDIES OF THE ROLE FOR DIHYDROPYRIMIDINASE RELATED PROTEIN 2 IN FORMATION OF EPILEPTIC SEIZURES IN EXPERIMENTAL MODEL AND CLINICS

Abstract

Article concerns results of the studies of the epileptic seizures carried out on the model of male rabbits of Chinchilla species and on the epileptic patients. In the 1 st series of studies after administration of 300 units of natrium penisillinum into the amygdala of the rabbits and induction of epileptic seizures blood samples were taken from them and with application of solid-phase indirect ELISA-test the level of dihydropyrimidinase-related protein 2 (DRP2) in the platelets and the level of anti-DRP2 autoantibodies in the serum were evaluated. During seizures downregulation of both DRP2 in the platelets (p<0.05), and anti-DRP2 autoantibodies in the serum (p<0.01) was revealed. In the 2 nd series of studies after achieving stable level of seizure activity in the rabbits, through cannula, earlier implanted into the brain lateral ventricle, DRP2 in a volume of 10 µL and concentration 0.7 mg/mL, in buffered saline solution (pH 7.3) was administered and 5 min later suppression of seizures was noted. In the 3 rd series of studies blood samples were taken from the veins of the patients having diagnosis of genuine epilepsy and with application of indirect ELISA- test the level of DRP2 in the platelets was measured and drastic, 3-fold upregulation of DRP2 (p<0.001) was revealed. The results presented in the article demonstrate prominent stabilizing role for serotonergic system and its sub-cellular signal proteins in regulation of excitation processes of brain cells and realization of inhibitory effects on formation of epileptic activity.