Abstract

The sequence of events in the infectious cycle of the endoparasite Bdello-vibrio bacteriovorus, from its attachment to the host surface to the release of progeny from lysed host are well established. However, the mechanisms involved in the parasite entry through a pore in the host cell wall into its periplasm have been topics for speculations but are not fully understood.Escherichia coli, Pseudomonas fluorescens and Spirillum serpens were infected by several Bdellovibrio strains (109, D and 6-5-S) in mixtures containing 109 to 1010 host cells/ml and host-parasite in ratios of 1:2 to 1:3, and were incubated at 30 C with shaking for 4 hr. At intervals specimens were prepared for electron microscopy and wet mounts were examined by phase optics.

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