Abstract
The 2-, 3-, 6-, 2′-, 3′-, 4′-, and 6′-deoxy derivatives and the 3- O-methyl derivative of methyl β-lactoside have been synthesised and their binding to the galactose-specific agglutinin from Ricinus communis (RCA-120) has been investigated. The results indicate that HO-3,4,6 of the β- d-galactopyranose moiety are the key polar groups. The main difference from the closely related ricin lectin RCA-60 involves HO-6 of the d-glucopyranose moiety, which seems to contribute to the binding of the carbohydrate to RCA-60 but not to RCA-120.
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