Abstract
The effects of five different intense sweeteners on the growth and metabolism of mixed cultures of dental plaque microorganisms or a pure culture of Streptococcus mutans NCTC 10449 were studied (a) in peptone liquid media, (b) in agar media by the zone inhibition method and (c) in tryptone soya medium by a modification of the minimum inhibitory concentration method. Acesulfam-K and cyclamate showed no activity. The effect of Talin was obscured by its tendency to precipitate from solution. Only aspartame and saccharin had any inhibitory action, with aspartame the more effective in the mixed plaque cultures and saccharin the more effective against S. mutans.
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