Studies of the inhibition of lipolytic enzymes: I. the inhibition of a canine kidney and liver lipase in vitro and in vivo BY n-butyl carbamic acid methyl ester (U-14641)

Abstract

n-Butyl carbamic acid methyl ester has been shown to be a potent inhibitor of canine liver and kidney lipases both in vitro and in vivo. The nature of this inhibition has been investigated and mechanisms consistent with the data are proposed. When this inhibitor is intravenously administered to dogs in suitable doses, a marked degree of inhibition of liver and kidney lipases has been demonstrated both by direct assay and by the greatly diminished rate of elimination of procaine from the plasma of dogs treated with this compound. A remarkable species specificity has been demonstrated, since butyl carbamic acid methyl ester does not inhibit liver and kidney lipases obtained from five other species.