Abstract
In the prevention of Toxoplasma gondii infection in man and animals, the inactivation of the parasite’s cysts in animal tissues and its oocysts in environment is essential. The objective of our studies was to extend our knowledge of the effects of various physical and chemical treatments on the viability of two stages of T. gondii. In several separate studies, we examined the effects of freezing, heating, and irradiation on the viability of tissue cysts, as well as the effects of freezing, heating, drying, four disinfectants [Aldesol, tincture of Hibisept, Izosan-G (all manufactured by PLIVA, Zagreb, Croatia) and Virkon-S (KRKA, Novo Mesto, Siovenia)], two alcohols, and 10% formalin on the viability of oocysts. In all our experiments, the viability of the treated and control tissue cysts and oocysts was assessed by bioassays on cats and/or mice. The cats were inoculated following the principles described by Dubey and Streitel (1976) and the mice were inoculated according to a protocol by Dubey at al. (1984).
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