Abstract
1. 1. The G + C content of the DNAs of Amoeba proteus and Amoeba indica was determined by buoyant density gradient centrifugation. 2. 2. Generally, different clones of the same strains did not differ in the G + C content of their main DNA or their satellite DNA. 3. 3. The G + C content of the main DNA of the T and XD strains of A. proteus were the same (25.3 and 29.3% respectively) as were the G + C contents of the satellite DNA (54.7 and 61.8% respectively). 4. 4. The main and satellite DNAs are reversed for the F strain of A. proteus and the I strain of A. indica; the G + C contents of the main DNA being 57.4 and 59.8% respectively and those of the satellite DNA being 23.5 and 28.4% respectively. 5. 5. The heterologous hybrids produced by nuclear transplantation between the A strain of A. proteus and the I strain of A. indica have the same G + C content in their main and satellite DNAs as do their cytoplasmic parents. 6. 6. The data suggest that the occurrence of phenotypic variations does not necessarily mean an alteration in the G + C content of either main or satellite DNA. 7. 7. There is also evidence that the G + C content of these DNAs is under cytoplasmic control.
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