Abstract
SUMMARY The concentration of sodium, potassium, calcium and magnesium in whole hlood, and the distribution of these cations between the erythrocytes and plasma of 6 species have been determined by atomic absorption spectrophotometry. The potassium and magnesium concentrations in human, bovine, sheep, dog, rabbit and fowl erythrocytes exceeded those of the plasma, while the reverse was true Jor sodium and calcium. In general, neither a rapid drop of temperature from 37°C to 0°C. nor a 1:2 dilution in sodium phosphate buffer caused marked movements of the cations into or out of the erythrocytes. There was no decrease in oxygen uptake, glucose utilization, or lactate accumulation of sheep and rabbit erythrocytes following cold shock. Dilution depressed the oxygen uptake of cold shocked sheep erythrocytes and caused a reduction in lactate production by rabbit erythrocytes.
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