Abstract
Daly & Mirsky (1949), using the method of Moore & Stein (1948), were the first to describe quantitative separation of six bases, thymine, uracil, cytosine, adenine, methylcytosine and hypoxanthine. dna was quantitatively isolated from fowl spermatozoa by Mirsky & Ris (1949) and Vendrely, Knoblock & Vendrely (1956). The object of this experiment was to identify and determine the base, nitrogen and phosphorus content of dna from fowl spermatozoa. Semen was collected from fifteen White Plymouth Rock males (18 months old) by the method of Burrows & Quinn (1937). Counts of spermatozoa in composite samples were made according to the haemocytometer technique used in counting red blood cells with a 1 : 100 dilution, dna was isolated according to Boone (1963). Isolated material was hydrolysed by heating in 5% sulphuric acid solution on a steam bath for 24 hr. To identify the nucleic acid base content, samples were subjected to hydrolysis by heating 5 mg of dna in 2-5 ml of 70% perchloric acid on the steam bath for 80 min (Lovetrup & Roos, 1963). Ion exchange chromatography was employed, using an Amberlite CG-120, a strong cation exchanger resin, column of 20 to
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