Abstract
A method is described for the preparation of luminescent extracts from the sea pansy, Renilla reniformis. Light production requires the presence of Renilla luciferase, an adenine-containing nucleotide (either ATP, ADP, or AMP), Renilla luciferin, and oxygen. The reaction is specific for adenine-containing nucleotides and Renilla luciferin. The luminescent reaction was shown to involve a nucleotide-dependent oxidation of Renilla luciferin. Various factors that affect the system were studied. These include the effect of pH, enzyme concentration, temperature, inhibitors, nucleotide concentration, and luciferin concentration. The evidence presented indicates that ATP is involved indirectly in this system by functioning as a generating system for ADP and AMP via an ATPase reaction.
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