Studies of the biological activity of LHRH analogs in the rainbow trout, landlocked salmon, and the winter flounder

Abstract

Studies of gonadotropic hormone (GtH) release bioactivity by mammalian and submammalian varities of LHRH and LHRH analog were primarily conducted in vivo in testosterone-primed yearling (TPY) rainbow trout, a convenient test animal for LHRH bioassays in fish. Validation of these results, using sexually mature fish, was accomplished by examining LHRH agonist activities on release of GtH in vivo in spermiating landlocked salmon and by studying LHRH peptide hormone binding affinities using a flounder pituitary LHRH radioreceptor assay. Our surveys of LHRH analog bioactivity in vivo in TPY trout and salmon demonstrated that all types offish, bird, and mammalian LHRH agonists possess superactive properties on the fish pituitary. The most active group of LHRH analogs, based upon both LHRH receptor binding affinity and in vivo release of gonadotropin, was judged to include [ d-Nal(2) 6,Pro 9-NHEt]LHRH, [ d-Nal(2) 6-AzaGly] 10]LHRH, [ d-Ala 6,Pro 9-NHEt]-LHRH, and the fish LHRH analogs, [ d-Arg 6,Trp 7 Leug,Pro 8-NHEt]LHRH, [ d-hArg(Et 2 6,Trp 7 LeU 8, Pro 9-NHEt]LHRH.