Studies of the Anti-Diabetic Mechanism of Pueraria lobata Based on Metabolomics and Network Pharmacology

Shu Zhang,Keping Chen,Qi Ge,Liang Chen

doi:10.3390/pr9071245

Abstract

Diabetes mellitus (DM), as a chronic disease caused by insulin deficiency or using obstacles, is gradually becoming a principal worldwide health problem. Pueraria lobata is one of the traditional Chinese medicinal and edible plants, playing roles in improving the cardiovascular system, lowering blood sugar, anti-inflammation, anti-oxidation, and so on. Studies on the hypoglycemic effects of Pueraria lobata were also frequently reported. To determine the active ingredients and related targets of Pueraria lobata for DM, 256 metabolites were identified by LC/MS non targeted metabonomics, and 19 active ingredients interacting with 51 DM-related targets were screened. The results showed that puerarin, quercetin, genistein, daidzein, and other active ingredients in Pueraria lobata could participate in the AGE-RAGE signaling pathway, insulin resistance, HIF-1 signaling pathway, FoxO signaling pathway, and MAPK signaling pathway by acting on VEGFA, INS, INSR, IL-6, TNF and AKT1, and may regulate type 2 diabetes, inflammation, atherosis and diabetes complications, such as diabetic retinopathy, diabetic nephropathy, and diabetic cardiomyopathy.

Highlights

Diabetes mellitus (DM), as a chronic disease caused by insulin deficiency or using obstacles, is gradually becoming a principal worldwide health problem
The roots of Pueraria lobata were purchased from Bozhou, Anhui Province, China, 2020, and the roots of the healthy plants picked in autumn were washed, dried, and in 2020, andground the roots of powder the healthy plants picked inSchool autumn were washed, to a fine in an electric grinder in the of Life
Yin deficiency in traditional Chinese medicine (TCM), which is usually associated with Qi deficiency (Qi and blood) and Yin deficiency

Summary

Introduction

Diabetes mellitus (DM), as a chronic disease caused by insulin deficiency or using obstacles, is gradually becoming a principal worldwide health problem. Pueraria lobata is one of the traditional. The results showed that puerarin, quercetin, genistein, daidzein, and other active ingredients in Pueraria lobata could participate in the AGE-RAGE signaling pathway, insulin resistance, HIF-1 signaling pathway, FoxO signaling pathway, and MAPK signaling pathway by acting on VEGFA, INS, INSR, IL-6, TNF and AKT1, and may regulate type 2 diabetes, inflammation, atherosis and diabetes complications, such as diabetic retinopathy, diabetic nephropathy, and diabetic cardiomyopathy. Diabetes mellitus (DM), including type 1, type 2, and gestational diabetes, is a chronic and metabolic disease with long-term hyperglycemia and complex pathogenesis, influenced by interactions of multiple factors such as genetics, environment, age, and sex. Traditional Chinese medicine (TCM) with low cost and stable efficacy has become a complementary and alternative medicine for treating DM [2,3]

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Conclusion