Abstract
The cDNA chimeras between two subtypes of mouse excitatory amino acid transporter family, mouse excitatory amino acid carrier 1 (mEAAC1) and mouse alanine serine cysteine transporter 1 (mASCT1), were constructed by recombinant PCR. After transcriptionin vitro, the cRNA was injected and expressed inXenopus laevis oocytes.3H-Glu and3H-Ser were used as isotopic tracer to measure the flux of amino acids. The results showed that there might not be the key amino acids responsible for substractive specificity in the NH2-terminal and its adjacent regions of these two transporters, which probably supported the formation of the substrate binding sites.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
