Studies of osteoblast-like MG-63 cellular proliferation and differentiation with cyclic stretching cell culture system on biomimetic hydrophilic layers modified polydimethylsiloxane substrate

Abstract

Cell culturing with physical stimulation has been in research focus lately because the human body and tissue are continuously receiving different mechanical stress in daily activities. Nevertheless, the study of the synergetic effect on cellular adhesion and proliferation by the dynamic stretching culture system on different surface grafted hydrophilic layers was rare. This study hypothesized the MG-63 cellular adhesion and proliferation will be affected by the surface chemistry under dynamic culturing. The PDMS cell culturing substrate was surface grafted with five common biocompatible materials, namely alginate, type I collagen, fibronectin, poly-l-lysine, and laminin. Results have indicated that neither surface charge nor surface hydrophilicity can be correlated well with the MG-63 cell adhesion and proliferation in the static or dynamic culture. The type I collagen grafted PDMS substrate in combination with appropriate physical stimulation showed excellent proliferation and expression of osteoblast associated proteins in MG-63, likely due to the adaption of the surface grafted layer to the extracellular environment. Moreover, the surface grafted type I collagen layer remained stable after ultrasonic cleaning. Henceforth, the combination of dynamic culture system and proper modification layer could be useful in selecting proper biomaterials for clinical uses as well as for future tissue/cell cultivation applications.