Abstract
The nucleosome is the universal protein/DNA element repeated throughout the chromatin of eukaryotic cells (Kornberg 1977; McGhee and Felsenfeld 1980). Presumably as a consequence of its fundamental role in the organization of DNA in a compact unit, it has also been found to take part in gene regulation (see, e.g., Pederson et al. 1986; van Holde 1988; Grunstein 1990; Wolffe 1991; Kornberg and Lorch 1992). A major objective of our laboratory has been to obtain structural information at high resolution for the nucleosome, its higher-order structure, and its interactions with other nuclear factors. Previously, the structure of the nucleosome core particle was solved by X-ray crystallography at 7 Å resolution using the method of multiple isomorphous replacement (Richmond et al. 1984). This X-ray structure confirmed the size and shape of the particle deduced earlier from several lines of information (Finch et al. 1979), showing that the “146”-bp DNA is arranged...
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