Studies of Lipid Monolayers Prepared from Native and Model Plant Membranes in Their Interaction with Zearalenone and Its Mixture with Selenium Ions

Elżbieta Rudolphi-Skórska,Barbara Gzyl-Malcher,Maria Filek,Apolonia Sieprawska

doi:10.1007/s00232-017-9958-x

Abstract

The impact of zearalenone and selenate ions on the monolayers of 1,2-dipalmitoyl-phosphatidylcholine (DPPC), 1,2-dipalmitoyl-3-trimethylammonium-propane (DPTAP), and the lipid mixtures (phospholipids and galactolipids) extracted from wheat plasmalemma has been studied using Langmuir trough technique and Brewster angle microscopy (BAM). The zearalenone is a mycotoxin that exerts toxic effects on the cells of plants and animals. Monolayers’ properties were characterized by surface pressure (π)—molecular area (A) isotherms. It was found that zearalenone interacts with lipid monolayers causing their expansion. The selenate ions, added to the subphase together with zearalenone, reduce the effect of this mycotoxin on the surface properties of lipid films.

Highlights

Monolayers, the most important model system in the investigations of the specific reactions between lipids, as well as the changes in their structure influenced by the Zearalenone (ZEA) (6-[10-hydroxy-6-oxo-trans-1-undecenyl]-B-resorcyclic acid lactone) with the molecular formula C18H22O5, known as RAL and mycotoxin F2, is a non-ionic compound produced by genus Fusarium in cereals (Duca et al 2006)
The degree of unsaturation was calculated as the ratio of molar content of unsaturated to saturated fatty acids (U/S)
On the basis of the research it can be stated that zearalenone can localize in the lipid monolayers, and the degree of penetration depends on their polarity

Summary

Introduction

Monolayers, the most important model system in the investigations of the specific reactions between lipids, as well as the changes in their structure influenced by the Zearalenone (ZEA) (6-[10-hydroxy-6-oxo-trans-1-undecenyl]-B-resorcyclic acid lactone) with the molecular formula C18H22O5, known as RAL and mycotoxin F2, is a non-ionic compound produced by genus Fusarium in cereals (Duca et al 2006). ZEA is a natural form of the trans isomer and an S configuration with its methyl group in position C3 (Kuo et al 1967) (Fig. 1). This mycotoxin is a non-steroidal substance, with the chemical structure similar to estrogen. ZEA is better soluble in alkaline solutions, alcohols, acetone, benzene, and chloroform. When it is present in small doses in plants, it shows activity similar to plant hormones in some physiological processes

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Discussion

Conclusion