Abstract
When a lysogen containing a heat-inducible λ mutant is subjected to an appropriate heat treatment, phage is usually produced and the bacterium dies. Under certain conditions, the heated bacterium dies although phage production does not take place. Non-lysogenic cells, and lysogens containing wild-type λ, do not die when heated. Therefore, death after heating must be due to an activity of the mutant prophage. In this study, killing of the lysogen is called induction, whether or not phage is produced.
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