Abstract
The precedinig papers of this series1' 2 repotted that the iinitial product, of bacterial DNA replicationi is isolated as low-molecular-weight single-stranided DNA. This first initermediate is converted, in the cell, into the second initermediate whose structure wheni isolated appears to be that of a double-stranlded DNA moiety cointaining a small but significant single-strainded portioin as well as gaps in the newly synthesized stranid. Okazaki and his associates showed that an appreciable amount of newly syinthesized DNA is isolated as single-stranded DNA.' An important question at this time conieerninig the conversion between these two different stages of DNA replication is whether an unknown enzyme(s) is, directly or indirectly, involved. As a first step in seeking to answer this question, strains of bacteriophage harboring conditional lethal mutations in genes implicated in DNA replication were examined. This paper reports that bacteriophage T4 strains which conitain coinditional mutations of gene 41, under nonpermissive conditions, accumulate newly synthesized DNA that is isolated as sin-gle-strainded DNA.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
More From: Proceedings of the National Academy of Sciences of the United States of America
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.