Studies of colicin action on wall-less stable L-forms of Escherichia coli. II. Growth inhibition of complete and wall-less (L-form) cells of Escherichia coli by basic colicin types.

Abstract

The inhibitive activity of colicins of 16 types (produced by 22 colicinogenic strains) on rods and protoplast-like stable L-form cells of the strains Escherichia coli B, W1655 F+ and W1655 F- was compared. The results of 58 combinations tested fall into four groups (with three subgroups): both cell forms are sensitive: a) both cell forms are about equally sensitive, b) rods are distinctly more sensitive than L-form cells, c) L-form cells are distinctly more sensitive than rods; only rods are sensitive, L-form cells are not sensitive; only L-form cells are sensitive, rods are not sensitive; neither cell form is sensitive. Group la represents simple sensitivity; both cell wall and cytoplasmic membrane receptors are present and functioning. Groups 1b and 2 represent sensitivity, substantially or completely mediated by cell wall receptors. Groups 1c and 3 represent partial or complete "pseudoresistance" or "pseudotolerance"; cell wall receptors are absent or non-lethal, but cytoplasmic membrane ones are present and mediate the lethal effect. Group 4 represents both true resistance and true tolerance.