Abstract
The interaction of several nucleosides with proflavine hemisulphate was investigated by absorption and fluorescence spectroscopy in solvents with different dielectric constants. In each case, the ground state charge transfer complex was formed (observed from the difference absorption spectra) and the formation constants were calculated using the Benesi-Hildebrand equation (H.A. Benesi and J.H. Hildebrand, J. Am. Chem. Soc., 71 (1949) 2703). Fluorescence spectroscopy ruled out the possibility of exciplex formation as no new band was observed. However, static quenching occurred within a limited range of acceptor to donor concentration ratio. The formation constants calculated were found to be higher than those observed by adsorption spectroscopy.
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