Abstract
Capillary electrophoresis (CE) is a unique method that can be used to directly observe the interaction among the molecules under conditions similar to physiological state without prior immobilization of the reactants to a solid phase. It is suitable in searching for carbohydrate-binding proteins and protein-binding carbohydrates. It also allows reliable micro/ultramicro-scale estimation of association/dissociation constants. Furthermore, CE enables direct observation of enzymatic reactions occurring in a capillary and assay of enzyme activity. This chapter describes the characteristic features of CE and shows how CE has been applied for various kinds of studies related to carbohydrate–protein binding. All these applications demonstrate the usefulness of the CE-based method, and the results obtained are considered to provide important information about various kinds of biological phenomena induced by carbohydrate–protein interaction.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
