Abstract
microscopy. This investigation was supported in part by fellowship 5-F2-CA-23, 140-02 from the National Cancer Institute, U. S. Public Health Service. * Based on a thesis submitted by the senior author to the graduate faculty of Cornell University in partial fulfillment of the requirements for the degree of Doctor of Philosophy. f Present address: Physical Sciences Division, United States Army Medical Unit, Walter Reed Army Medical Center, Fort Detrick, Frederick, Maryland 21701. broth supplemented with 10*^ swine serum, prepared according to Barber and Fabricant (1962). Occasionally, to visualize individual colonies agar media corresponding to these broths were used, also as described by Barber and Fabricant. All cultures were incubated aerobically in a moist environment at 37 C. Electron microscopy.?Blocks approximately 3 mm on a side were cut from areas of confluent Mycoplasma growth on 4-day-old agar cultures and from selected infected and uninfected tissues. These blocks were fixed for 1
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