Abstract
The interaction and binding abilities between the trans-activation response (TAR) element, residues 1–59 of the human immunodeficiency virus type 1 (HIV-1) mRNA, and four computer-simulated designed potentially small-molecule drugs were studied by capillary zone electrophoresis (CZE). Experimental results indicated that these computer-simulated small-molecule drugs could specifically bind to TAR RNA and effectively inhibit the complex formation of HIV-1 trans-activator of transcription (Tat) protein to TAR RNA – an essential component for HIV-1 transcription. The small molecular inhibitors and TAR RNA could be baselinely separated for each individual drug under optimized CE experimental conditions. Their binding constants were quantitatively determined and the data were used for the drug evaluation and screen in lead discovery.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.