Abstract

1.1. O2 uptake by rat-heart cells decreases with time in culture.2.2. Primary cells have a respiratory quotient of 0.81, which indicates mainly fat metabolism, while cultured cells have a higher respiratory quotient of 0.96, indicating a shift from fat to carbohydrate metabolism.3.3. Both Crabtree and Pasteur effects are established after 1 week in culture, indicating development of a control mechanism centered about glucose metabolism in culture.4.4. Glucose and palmitate stimulate O2 uptake of primary cells but lose their stimulatory abilities when the Crabtree effect appears. Pyruvate stimulates O2 uptake of both primary and cultured cells to the same extent.5.5. Amytal, at a concentration of 1 mM, inhibitsO2 uptake of primary cells and this inhibition increases with time in culture. In primary cells increased inhibition of O2 uptake by Amytal is obtained in the presence of glucose, despite the fact that addition of glucose to non-inhibited cells increase O2 uptake, while addition of palmitate to Amytal-inhibited cells increases O2 uptake.6.6. A possible shift from fat to carbohydrate metabolism in cultured rat-heart cells has been discussed.

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