Abstract
The nutritional requirements of Corynebacterium sepedonicum (Spiek. and Kott.) Skapt. and Burkh. were investigated by the use of the Warburg respiration apparatus. Pantothenic acid, in a concentration of 600 micrograms (μgm.) per liter, caused the greatest growth augmentation of nine vitamins tested. The amino acids, glutamic acid, asparagine, leucine, and lysine in concentrations of 0.001, 1. 0, 0.03, and 0.03 gm. per liter respectively, caused a marked increase in oxygen uptake, either individually or in combination. Yeast nucleic acid and thymonucleic acid had no significant effect on the growth rate of C. sepedonicum. A new medium was prepared for the isolation and maintenance of C. sepedonicum.
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