Studies in Experimental Eosinophilia

Abstract

Abstract Puromycin blocked the eosinophil response which is elicited in guinea pig lymph nodes by a single injection of hemocyanin into footpads. The drug was most effective in interfering with the 24-hr response when it was given just before or no later than 4 hr after the antigen. A partial block was noted when the drug preceded the antigen by 2 to 8 hr. Interference with the 15-min vascular response required a higher dose. The secondary type of eosinophil response, which is seen in an immunized animal, was not inhibited by puromycin. The drug was effective when administered into the footpads, but a higher dose was required when it was given by the intraperitoneal route. The results support the notion that the eosinophilia which occurs during the first few minutes of a primary immune response reflects the de novo synthesis of antibody, rather than the presence of preformed antibody.