Abstract
The study of chromatogram peak pattern by influence of excipient in paracetamol tablet with TLC- Spectrophotodensitometry method for drug profiling has been done. Sample (6 kind of paracetamol tabletsfrom different factory) and reference compounds were analyzed with TB (cyclohexane : toluene:diethylamine = 75:15:10) and TAEA (toluene : acetone : ethanol: ammonia = 45:45:7:3) as mobile phase andAl-TLC Si 60 G F254 as stationary phase.The initial step was non analyte peak reduction on Al-TLC plate with washing method, drying time and temperature variation. Sample was extracted with 1 ml chloroform and Na2CO3 buffer pH 10 and spotted onAL-TLC plate. First, sample extract from same factory was spotted on one plate, continued by sample extractfrom 6 different factories on one plate which is replicated five fold on each plate that different. Plate was eluted and scanned at 210 nm. Chromatogram was analyzed by cosine and cluster function. C95 value thatwas calculated from sample was used as a parameter to determine the influence of excipient towardchromatogram peak pattern.The result of this research showed that each tablet from same factory gave C95 value near 100 if calculated with cosine function (96 - 98,7 for TB and 78 - 83 for TAEA), whereas if all tablets from 6different factories was used, the excipient from each tablet will give C95 value alteration (63 for TB and 68 for TAEA). Cluster analysis will divide sample into 3 different groups for TB and 1 group for TAEA.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
More From: Indonesian Journal of legal and Forensic Sciences
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.