Abstract
Sarimana U, Indarto N, Herrero J, Erika P, Wendra F, Zulhermana Z, Asmono D. 2019. Genetic diversity preliminary study of cameroon origin oil palm at PT. Binasawit Makmur. Jurnal Lahan Suboptimal: Journal of Suboptimal Lands. 8(2):126-133. A plant breeding program needs genetic diversity information of germplasm, but the source of genetic diversity of oil palm plants in Indonesia is very narrow. In order to increase genetic diversity, the introduction of germplasm from Cameroon was conducted. The purpose of this study was to obtain a useful molecular marker in order to get genetic diversity information in Elaeis guinensis germplasm materials from Cameroon at PT. Binasawit Makmur (BSM). This preliminary research has been done in 2013. In this study, 300 samples of Cameroon oil palm DNA from 99 accessions and 10 samples DNA BSM material were analyzed by 9 microsatellite markers (simple sequence repeat; SSR). The 9 SSR markers used were very informative and we obtained 93 alleles with an average of 10.33 alleles per locus. The value of polymorphism information content (PIC) was around 0.746-0.889. The total genetic diversity was relative high (HT = 0.814). The coordinate analysis showed no groups in both 99 accessions and BSM material. Based on phylogenetic analysis, 9 SSR markers could show the genetic diversity between the populations. To validate this fact, it needs further research using more molecular markers and samples.
Highlights
Program pemulian tanaman sangat membutuhkan informasi tentang keragaman genetik plasma nutfah, akan tetapi diversitas genetik tanaman kelapa sawit di Indonesia sangat rendah
Topologi analisis dilakukan dengan menggunakan metode neighbour-joining clustering dengan 100 bootstrap replika dan Unweighted Pair-Group Method Arithmetic Average (UPGMA), menggunakan Sequential Agglomerative Hierarchical Nested Cluster Analysis (SAHN-clustering) dan TREE program from NTSYS-pc, version 2.02 packages (Rohlf 1998) dan DARwin 6 (Soft ware developed by CIRAD berdasarkan Dice index (Nei 1983)
Allelic diversity of natural oil palm (Elaeis guineensis Jacq.) populations detected by microsatellite markers: implications for conservation
Summary
Pokok-pokok ini merupakan hasil dari eksplorasi di Kamerun (Centre, East, Littoral, Northwest, South, Southwest, West). Lokasi sampling dicatat masing-masing berdasarkan Global Positioning System dengan variasi ketinggian antara 9-1326 m dpl (Gambar 1 peta lokasi sampling) dan jumlah pokok yang digunakan (Tabel 1). 3017 pokok ini, kemudian dipilih 300 pokok yang digunakan dalam preliminary study ini. Pemilihan sample ini diambil secara acak mewakili ke 99 aksesi yang ada. Jumlah aksesi dan jumlah pokok yang digunakan sebagai sample pada tiap wilayah
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