Struktur und Funktion der genetischen Information in den Plastiden: XIII. Lamellarproteine bleicher Plastiden von Plastom- und Genmutanten von Hordeum und Lycopersicon

Abstract

Summary The plastid lamellar proteins of two plastom mutants of Hordeum vulgare and two gene mutants of Lycopersicon esculentum were investigated by electrophoresis in Polyacrylamide gels. The plastidal protein synthesis in the plastom mutants of the mutant lines “Saskatoon” and “albostrians” og Hordeum is impaired The deficiency of plastidal protein synthesis causes distinct changes in the pattern of the lamellar proteins as compared with the green control. The chlorophyll-protein complex I is absent. But numerous lamellar proteins are found in the white mutant leaves. These proteins — among them presumably also the protein of the chlorophyll-protein complex II — are expected to be synthesized on cytoplasmic ribosomes. In the gene mutants albina-2 and sulfureapura of Lycopersicon the chlorophyll-protein complex I is also absent. These results suggest that the formation of the chlorophyll-protein complex I is not only controlled by the genetic information of the plastids (plastom), but also by the genetic information of the nucleus (genome). Two reasons for the absence of the chlorophyll-protein complex I in these different mutant are discussed: (1) The protein component of the chlorophyll-protein complex I consists of difieren proteins of lower molecular weight, coded partly by the genome, partly by the plastom and synthesized on cytoplasmic and on plastid ribosomes, respectively. (2) The protein of the chlorophyll-protein complex I will not be synthesized and/or will not be incorporated into the plastid lamellar structure before the differentiation of the chloroplast has reached a particular stage.