Structures of the O chains from lipopolysaccharides of Campylobacter jejuni serotypes O:23 and O:36

Abstract

Lipopolysaccharides of C. jejuni serotypes O:23 and O:36 have been shown to contain structurally variable O polysaccharide chains with repeating units of four closely-related types: → 3)-β- d-Glc pNAc-(1 → 3)-α- d-Gal p-(1 → 2)-6d-α- d- alt-Hep p-(1 → → 3)-β- d-Glc pNAc-(1 → 3)-α- d-Gal p-(1 → 2)-6d-3-Me-α- d- alt-Hep p-(1 →, → 3)-β- d-Glc pNAc-(1 → 3)-α- d-Gal p-(1 → 2)- d- glycero-α- d- alt-Hep p-(1 →, and → 3)-β- d-Glc pNAc-(1 → 3)-α- d-Gal p-(1 → 2)-3-Me- d- glycero-α- d- alt-Hep p-(1 →. Structural methods included 1H- and 13C-NMR spectroscopy, methylation linkage analysis, fast atom bombardment mass spectrometry of methylated glycans, and selective fragmentations by the Smith degradation and N-deacetylation-nitrous acid deamination.