Structures of the K35 and K15 capsular polysaccharides of Acinetobacter baumannii LUH5535 and LUH5554 containing amino and diamino uronic acids

Alexander S Shashkov,Bin Liu,Johanna J Kenyon,Anastasiya V Popova,Mikhail M Shneider,Sof'Ya N Senchenkova,Nikolay P Arbatsky,Konstantin A Miroshnikov,Lei Wang,Yuriy A Knirel

doi:10.1016/j.carres.2017.05.017

Structures of the K35 and K15 capsular polysaccharides of Acinetobacter baumannii LUH5535 and LUH5554 containing amino and diamino uronic acids

Alexander S Shashkov, Bin Liu + Show 8 more

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https://doi.org/10.1016/j.carres.2017.05.017

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Journal: Carbohydrate research	Publication Date: May 25, 2017
Citations: 30	License type: cc-by-nc-nd

Affiliation: N.D. Zelinsky Institute of Organic Chemistry, Nankai University, University of Technology, University of Sydney, Moscow Institute of Physics and Technology, State Research Center for Applied Microbiology and Biotechnology, Institute of Bioorganic Chemistry

#O-acetyl Groups #1D 1H NMR Spectroscopy + Show 8 more

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Abstract

Capsular polysaccharides were isolated from A. baumannii LUH5535 (K35 CPS) and LUH5554 (K15 CPS) and studied by 1D and 2D 1H and 13C NMR spectroscopy. The CPSs were found to consist of linear tetrasaccharide repeats (K units) containing 2-acetamido-2-deoxy-d-galacturonic acid (K35) or 2,3-diacetamido-2,3-deoxy-d-glucuronic acid (K15) and 2,4-diacetamido-2,4,6-trideoxy-d-glucose (both CPSs). The K35 unit includes three O-acetyl groups on different GalNAcA residues. A. baumannii LUH5535 and LUH5554 carry the KL35 and KL15 gene clusters, respectively, and putatively assigned functions of genes in these clusters were consistent with the CPS structures established.

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