Structures of nitroaromatic compounds induce Shewanella oneidensis MR-1 to adopt different electron transport pathways to reduce the contaminants

Abstract

Nitroaromatic compounds (NACs) are one class of typical refractory biodegradable organic pollutants detected in various environmental media. The reductive transformation of NACs by the electrochemically active bacterium Shewanella oneidensis MR-1 is a possible and cost-effective option for the removal of NACs. However, little information on the respiratory pathway employed by S. oneidensis MR-1 to reduce NACs is available. In the present study, we investigated the reduction of NACs with different nitro or alkyl moieties by S. oneidensis MR-1 and eight constructed mutants with the deletion of the mtrA, mtrB, mtrC/omcA, cymA, napA, napB, nrfA, and nfnB genes to explore these key functional enzymes. The reduction of nitrobenzene, 4-nitrotoluene, 4-ethylnitrobenzene, 1-tert-butyl-4-nitrobenzene, 1,2-dinitrobenzene, 1,3-dinitrobenzene, 1,4-dinitrobenzene, 2,4-dinitrotoluene, 2,6-dinitrotoluene and 2,4,6-trinitrotoluene occurs via both the Mtr respiratory pathway and NfnB related pathway. However, 2,5-ditertbutyl-nitrobenzene, 2-nitrobiphenyl and 2,2′-dinitrobiphenyl are reduced only via the Mtr respiratory pathway. The van der Waals volume of NACs was the key factor in determining the reduction by S. oneidensis MR-1 according the correlation analysis. Our study provides new insights into the environmental adaption of S. oneidensis MR-1 and facilitates the bioremediation of NAC-related contamination.