Abstract
This chapter focuses on the application of Raman spectroscopy to probe structures and environments of complex biomolecules. It discusses the fundamental processes of Raman and resonance Raman (RR) scattering, followed by a presentation of the experimental considerations for this technique. A brief discussion on the principles of Raman spectroscopy is presented. The chapter illustrates the type of information that may be learned from UV RR (UVRR) studies of peptides in the context of folding and interactions with membranes. The combination of model compounds and development of library of spectral markers makes UVRR a valuable method to probe conformational changes in biomolecules. An inherent advantage of this vibrational technique is that it provides molecular information of biomolecules and reveals site-specific information about backbone and aromatic residues, including noncovalent interactions. The chapter presents examples of applications of RR spectroscopy and concludes with a discussion of the challenges and benefits of this method.
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