Abstract
.Structured light imaging (SLI) with high spatial frequency (HSF) illumination provides a method to amplify native tissue scatter contrast and better differentiate superficial tissues. This was investigated for margin analysis in breast-conserving surgery (BCS) and imaging gross clinical tissues from 70 BCS patients, and the SLI distinguishability was examined for six malignancy subtypes relative to three benign/normal breast tissue subtypes. Optical scattering images recovered were analyzed with five different color space representations of multispectral demodulated reflectance. Excluding rare combinations of invasive lobular carcinoma and fibrocystic disease, SLI was able to classify all subtypes of breast malignancy from surrounding benign tissues () based on scatter and color parameters. For color analysis, HSF illumination of the sample generated more statistically significant discrimination than regular uniform illumination. Pathological information about lesion subtype from a presurgical biopsy can inform the search for malignancy on the surfaces of specimens during BCS, motivating the focus on pairwise classification analysis. This SLI modality is of particular interest for its potential to differentiate tissue classes across a wide field-of-view () and for its ability to acquire images of macroscopic tissues rapidly but with microscopic-level sensitivity to structural and morphological tissue constituents.
Highlights
Breast-conserving surgery (BCS) involves removing the mass of tumor along with a surrounding layer of healthy tissue margin
BCS has been shown to be as effective as a full mastectomy for treating early-stage cancers, provided that the margins are clear of malignancy.[1]
Better methods for intraoperative margin assessment are of growing interest because 15%–35% of patients require a second surgery due to positive margins resulting from their initial BCS procedure.[4,5,6,7,8]
Summary
Breast-conserving surgery (BCS) involves removing the mass of tumor along with a surrounding layer of healthy tissue margin. For ductal carcinoma in situ (DCIS), a 2-mm zone of malignancy-free tissue should exist on all sides of a resected specimen for its margins to be considered clear, with DCIS distances less than 2 mm but not “on ink” reserved for clinical judgment.[2,3] Better methods for intraoperative margin assessment are of growing interest because 15%–35% of patients require a second surgery due to positive margins resulting from their initial BCS procedure.[4,5,6,7,8] Fast, effective detection of involved margins at the time of the first operation would save costs and reduce morbidity associated with a second procedure.
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
