Abstract
Phytase is a kind of enzyme that hydrolyzes phytic acid and its salts to produce inositol and phosphoric acid. As a new feed additive, phytase has great potential in animal nutrition and environmental protection. Because of its good stability, large-scale production and high activity, microbial phytase has become a hot spot in industrial application. Here, we reported the predicted structure and enzymatic properties of a phytase from Bacillus subtilis, which was named as phyS. It was clear that the optimal temperature is 35°C, and the optimal pH is 8. Meanwhile, the enzyme activity was kept at above 90% in the range of pH 8 - 9, this result demonstrated that phyS is an alkaline phytase. This study lays a foundation for the extensive application of phyS.
Highlights
Feed industry is one of the top ten basic industries in China’s national economy
Because of its good stability, large-scale production and high activity, microbial phytase has become a hot spot in industrial application
Phytase has a special spatial structure, which can separate phosphorus from phytic acid molecules in turn, degrade phytic acid into inositol and inorganic phosphorusand release other nutrients combined with phytic acid, which can be used for animal growth and development [2]
Summary
Feed industry is one of the top ten basic industries in China’s national economy. Feed enzyme preparation has attracted more and more attention because it is widely used in the fields of improving the production performance of animals and controlling the pollution of excrement [1]. Phytase has a wide range of sources It is found in plants, animals, fungi, yeast and bacteria. Due to the characteristics of microbial phytase, such as wide range of action, good stability, easy to scale production, high activity and short growth cycle, it has become the focus of research [3]. BPP has a special folding bucket structure in its molecular structure, so it gets its name This kind of phytase was first found in Bacillus [7], most of the BPP found at present still comes from Bacillus [8] [9] [10] which is the only neutral partial alkaline phytase (pH 6.0 - 8.0) in the phytase. Obtaining BPP with higher enzyme activity is the urgent task for its commercial production
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