Abstract
Structure of the quaternary complex of histone H3-H4 heterodimer with chaperone ASF1 and the replicative helicase subunit MCM2.
Highlights
The building block of eukaryotic chromatin is the nucleosome core particle (NCP), which is consisted of ∼146 bps of DNA wrapped around an octamer of core histones
The H3-H4 heterodimer is centrally located in the quaternary complex, enclosed on one side by MCM2 and the opposite side by ASF1 (Fig. 1B)
It is interesting to note that while βC is positioned in our quaternary complex and the ASF1-H3-H4 ternary complex, it contrasts sharply with that found in the (MCM2-H3-H4)2 complex (Fig. 1E)
Summary
The building block of eukaryotic chromatin is the nucleosome core particle (NCP), which is consisted of ∼146 bps of DNA wrapped around an octamer of core histones. A comparison with the recently published structure of the (MCM2-H3-H4)2 complex (PDB code: 4UUZ) reveals that MCM2 interacts with H3-H4 in the two complexes (Fig. 1C) (Richet et al, 2015).
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