Abstract
The genome of Mengo virus, “35 S” RNA, is a single polynucleotide chain having a molecular weight of 2.44 ± 0.08 × 10 6 daltons. This value has been determined from studies using the methods of polyacrylamide gel electrophoresis, sedimentation in dimethyl sulfoxide, sedimentation after reaction with formaldehyde and electron microscope measurements of the viral replicative form. SDS-polyacrylamide gel electrophoresis of solubilized purified virus revealed the presence of four major polypeptides (α, β, γ, and δ) having estimated molecular weights of 32,500, 29,600, 23,700, and 10,600 daltons, respectively. In addition, two minor components were detected. The first (D2) has a molecular weight of 57,700 daltons and the second (ϵ), a molecular weight of 39,000 daltons.
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