Abstract
The crystal structure of a conserved hypothetical protein, GK0453, from Geobacillus kaustophilus has been determined to 2.2 Å resolution. The crystal belonged to space group P4(3)2(1)2, with unit-cell parameters a = b = 75.69, c = 64.18 Å. The structure was determined by the molecular-replacement method and was refined to a final R factor of 22.6% (R(free) = 26.3%). Based on structural homology, the GK0453 protein possesses two independent binding sites and hence it may simultaneously interact with two proteins or with a protein and a nucleic acid.
Highlights
As part of the RIKEN Structural Genomics Initiative (RSGI) project, in collaboration with UK Structural Genomics, we selected the hypothetical protein GK0453 (13 kDa, 113 residues) from Geobacillus kaustophilus HTA426 to predict its function from analysis of its crystal structure
We describe the crystal structure of the hypothetical DUF1811-family protein GK0453 from G. kaustophilus and discuss its function based on structural homology
The tobacco etch virus (TEV) protease recognition sequence was inserted in the N-terminal tag region of the expression vector, which was introduced into the Escherichia coli Rosetta (DE3) strain (Merck Novagen, Darmstadt, Germany)
Summary
As part of the RIKEN Structural Genomics Initiative (RSGI) project, in collaboration with UK Structural Genomics, we selected the hypothetical protein GK0453 (13 kDa, 113 residues) from Geobacillus kaustophilus HTA426 to predict its function from analysis of its crystal structure. G. kaustophilus, from the Bacillaceae family, was isolated from deepsea sediment from the Mariana Trench (Takami et al, 1997). It is an aerobic, endospore-forming, Gram-positive bacterium that grows optimally at 333 K, with an upper temperature limit of 347 K (Takami et al, 2004). The crystal structure of YfhH from B. subtilis, which belongs to this family, has been determined (PDB entry 1sf; Midwest Center for Structural Genomics, unpublished work); the function of this protein is still unclear. We describe the crystal structure of the hypothetical DUF1811-family protein GK0453 from G. kaustophilus and discuss its function based on structural homology
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: Acta crystallographica. Section F, Structural biology and crystallization communications
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
