Abstract
TRiC is a cytoplasmic chaperonin involved in actin and tubulin folding. It is formed by six to nine different but related proteins of 52 to 65 kDa arranged in two hetero-oligomeric rings. We have cloned the gene coding for the mouse TRiC-P5 subunit (also called CCTγ) using aXbaI–DraIII fragment of the mTRiC5 cDNA. The mouse genome contains oneTRiC5gene and oneTRiC5pseudogene located on chromosomes 3F and 5B, respectively. The 2-kb transcript ofTRiC5is encoded by 14 exons distributed within 25 kb of genomic DNA. The largest exon is 312 bp and the smallest exon is 51 bp. We have used primer extension to demonstrate multiple transcription start points for theTRiC5gene. This is consistent with the lack of any obvious TATA box upstream of the transcription start points.
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