Structure of the GDP–Pi complex of Gly203 →Ala G iα1: a mimic of the ternary product complex of Gα-catalyzed GTP hydrolysis

Abstract

Background G proteins play a vital role in transmembrane signalling events. In their inactive form G proteins exist as heterotrimers consisting of an α subunit, complexed with GDP and a dimer of β γ subunits. Upon stimulation by receptors, G protein α subunits exchange GDP for GTP and dissociate from β γ . Thus activated, αsubunits stimulate or inhibit downstream effectors. The duration of the activated state corresponds to the single turnover rate of GTP hydrolysis, which is typically in the range of seconds. In G iα1, the Gly203→Ala mutation reduces the affinity of the substrate for Mg 2+, inhibits a key conformational step that occurs upon GTP binding and consequently inhibits the release of β γ subunits from the GTP complex. The structure of the Gly203→Ala mutant of G iα1 (G203AG iα1) bound to the slowly hydrolyzing analog of GTP (GTP γS) has been determined in order to elucidate the structural changes that take place during hydrolysis. Results We have determined the three dimensional structure of a Gly203→Ala mutant of G iα1 at 2.6 å resolution. Although crystals were grown in the presence of GTP γS and Mg 2+, the catalytic site contains a molecule of GDP and a phosphate ion, but no Mg 2+. The phosphate ion is bound to a site near that occupied by the γ-phosphate of GTP γS in the activated wild-type α subunit. A region of the protein, termed the Switch II helix, twists and bends to adopt a conformation that is radically different from that observed in other G iα1 subunit complexes. Conclusions Under the conditions of crystallization, the Gly203→Ala mutation appears to stabilize a conformation that may be similar, although perhaps not identical, to the transient ternary product complex of G iα1-catalyzed GTP hydrolysis. The rearrangement of the Switch II helix avoids a potential steric conflict caused by the mutation. However, it appears that dissociation of the γ-phosphate from the pentacoordinate intermediate also requires a conformational change in Switch II. Thus, a conformational rearrangement of the Switch II helix may be required in Gα-catalyzed GTP hydrolysis.