Abstract
The 0104 antigen (lipopolysaccharide, LPS) of Escherichia coli has an acidic O specific polysaccharide. From the aqueous phase of a phenol water extraction of E. coli O104 : K − , a fraction was obtained by ultracentrifugation and Cetavlon precipitation of the supernatant, which was enriched in long-chain LPS. Compositional analysis, NMR spectroscopy, periodate oxidation and methylation analysis showed that the polysaccharide chain of O104 LPS II consisted of galactose, N- acetylgalactosamine and neuraminic acid and acetate in the molar ratio of 2 : 1 : 1 : 1 and contained 3-βGal, 3-βGalNAc, 4-αGal, and 4-α(9-OAc-NeuNAc) in linear sequence. The same results were obtained with the capsular K9 polysaccharide from E. coli O9 : K9 , as presented here and reported previously (Dutton et al. (1987) Carbohydr. Res. 170, 193–206).
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