Structure of the d-mannan of the pathogenic yeast, Candida stellatoidea ATCC 20408 (Type II) strain, in comparison with that of C. stellatoidea ATCC 36232 (Type I) strain

Abstract

Acid treatment of the cell-wall d-mannans of Candida stellatoidea strains ATCC 36232 (Type I, A3 strain) and ATCC 20408 (Type II, A2 strain) gave (1 → 2)-linked β- d-manno-oligosaccharides (dp 2–5), whereas treatment with alkali gave the (1 → 2)-linked α- d-mannobiose. Conventional acetolysis of the acid- and alkali-treated d-mannan of the A3 strain gave oligosaccharides consisting of (1 → 2)- and (1 → 3)-linked α- d-mannopyranose residues, similar to those of Candida albicans serotype B strain. Mild acetolysis of the acid- and alkali-treated d-mannan of the A2 strain gave higher oligosaccharides that were digested by the Arthrobacter GJM-1 strain exo-α- d-mannosidase. The results of 1H- and 13C-NMR analyses indicated this d-mannan to contain branches with the following structures; β- d-Man p-(1 → 2)-α- d-Man p-(1 → 2)-α- d-Man p-Man p-(1 → 2)- d-Man. β- d-(1 → 2)-β- d-Man p-(1 → 2)-α- d-Man p-(1 → 2)-α- d-Man p-(1 → 2)-α- d-Mann p-(1 → 2)- d-Man, and β- d-Man p-(1 → 2)-β- d-Man p-(1 → 2)-β- d-Man p-(1 → 2)-α- d-Man p-(1 → 2)-α- d-Man p-(1 → 2)-α- d-Man p-(1 → 2)- d-Man, in common with the d-mannans of C. albicans serotype A strains.