Abstract
The structure of the d-mannan of Candida stellatoidea IFO 1397 strain, which has properties identical to those of the phospho- d-mannan of C. albicans serotype B strain, does not contain phosphate groups, and its 1H- and 13C-n.m.r. spectra are quite similar to those of the phospho- d-mannan of C. albicans NIH B-792 strain. However, the 1H-n.m.r. and 1H- 13C-correlation n.m.r. spectra of the products obtained by digestion with a- d-mannosidase of C. stellatoidea d-mannan considerably differed from those of the corresponding digestion products of the C. albicans phospho- d-mannan. Additionally, the enzyme-linked immunosorbent assay, by means of a monoclonal antibody corresponding to (1→2)-linked β- d-oligomannosyl residues, of the phospho- d-mannan of the same C. albicans strain indicated that the C. stellatoidea d-mannan does not contain any (1→2)-linked β- d-oligomannosyl residues. The absence of these residues may be used as one of the criteria of chemotaxonomical identification of C. stellatoidea spp.
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