Abstract
Lipopolysaccharides were isolated from Pseudomonas aeruginosa PAC1R and its lipopolysaccharide-defective mutants. Core fractions were obtained from them following mild acid hydrolysis, by gel chromatography using Bio-Gel P6. The core fraction from the parent strain contained glucose, rhamnose, galactosamine, heptose and alanine in the approximate molar ratios 3:1:1:1:1 while core fractions from the defective mutants lacked rhamnose and/or one, two, or three glucose units. Methylation analysis of the core fractions including identification of the partially methylated alditol acetates by combined gas-liquid chromatography and mass spectroscopy allowed the formulation of possible structures. These were confirmed by 13C nuclear magnetic resonance spectroscopy. Fractions consisting of core to which was attached unpolymerised O-antigen were isolated from the parent strain and one of the mutants and analysed in the same way to identify the attachment point of the O-antigen. The combined data leads to the formulation of the core oligosaccharide of PAC1R and its mutants as follows:
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