Abstract
The composition and structure of cell wall polysaccharides of a biotechnologically promising probiotic strain of bifidobacteria Bifidobacterium bifidum BIM B-465 were established by monosaccharide and methylation analyses along with 2D 1H– 1H homonuclear and 1H– 13C heteronuclear correlation NMR spectroscopy. The major polysaccharide represents a branched glucogalactan consisting of heptasaccharide repeating units having the following structure: [Display omitted] The second isolated polysaccharide is a branched glucan with the main chain of (1→6)-linked α- d-glucopyranose residues, ∼60% of which are 2-substituted with a single α- d-glucopyranosyl group. This polysaccharide is a characteristic of various microorganisms, including some lactobacteria studied earlier.
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