Abstract
A capsular polysaccharide was obtained from Alteromonas nigrifaciens IAM 13010T by saline extraction. On the basis of 1H and 13C NMR spectroscopy, including one-dimensional (1D) NOE spectroscopy, 2D rotating-frame NOE spectroscopy (ROESY), and 1H-detected heteronuclear 1H, 13C multiple-quantum coherence (HMQC), it was concluded that the polysaccharide contained inter alia an acidic sugar, 3-deoxy- d- manno-octulosonic acid (Kdo), and a rare amino sugar, 2-acetamido-2,6-dideoxy- l-talose ( l-6dTa1NAc, N-acetylpneumosamine), and has a pentasaccharide repeating unit of the following structure: ▪
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