Abstract
The most direct and informative probe of ribosome structure is electron microscopy (EM), of negatively stained and antibody-labelled specimens. Ribosomes from Escherichia coli have been the most intensively studied, although those from other prokaryotes and eukaryotes have also been imaged [e.g., 3, 4, 5],The amount of structural information obtained from electron images can be significantly increased using multivariate statistical analysis and classification techniques [e.g., 6]. Noisy electron micrographs of single particles are sorted according to their principal features, and average images formed with an enhanced signal-to-noise ratio and better reproducible resolution. The class averages represent projections from which a three-dimensional reconstruction can be computed.Thermus aguaticus is a species of extremely thermophilic bacteria isolated from environments such as hot springs. This species possesses macromolecular enzyme complexes with a great thermostability which must be reflected somehow instructural differences. In this study, we investigate the structures of ribosomes and ribosomal subunits from this bacterium.
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More From: Proceedings, annual meeting, Electron Microscopy Society of America
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