Structure of prodigiosin from Serratia marcescens NJZT-1 and its cytotoxicity on TSC2-null cells

Yinjuan Zhao,Fanxi Peng,Yan Xu,Jingkai Zhao,Ben Fan,Yuting Cao,Bin Xue,Zizhu Shen,Qi Cheng

doi:10.1590/fst.35719

Abstract

Prodigiosin, a secondary metabolite extracted from Serratia marcescens (S. marcescens), could induce apoptosis in various cancer cells, with however low toxicity on normal cells. The red pigment was extracted from a strain S. marcescens NJZT-1 isolated from soil, which had antibacterial activity. Spectral analyses (LC-ESI-MS, UV-VIS spectrophotometry, infrared spectra and HPLC) and TLC indicated the presence of prodigiosin in the extracellular bacterial culture extracts. The red pigment effectively killed the TSC2-null cells, whose mutation resulted in the progressive and systemic disease LAM. Our findings provide interesting evidence and important basis for the development of new therapeutic compounds with high potential effects against TSC2-null cells.

Highlights

Prodigiosin is a secondary metabolite alkaloid with unique tripyrrole chemical structure, which is a red pigment isolated from Serratia, Pseudomonas and Streptomyces (Casullo de Araújo et al, 2010; Sam & Ghoreishi, 2018; Younis et al, 2016)
To obtain an appropriate amount of bacterial biomass and pigment, the strain was transferred into a 1000-mL Erlenmeyer flask, containing 300 mL Luria Bertani (LB) broth medium, which was incubated at 28 °C, pH 7.2 for 72 h at 200 rpm
The S. marcescens NJZT-1 strain was isolated, with the temperature-stability according to the previous study, and the red pigment could be synthesized at 37 °C

Summary

Introduction

Prodigiosin is a secondary metabolite alkaloid with unique tripyrrole chemical structure, which is a red pigment isolated from Serratia, Pseudomonas and Streptomyces (Casullo de Araújo et al, 2010; Sam & Ghoreishi, 2018; Younis et al, 2016). Prodigiosin has been used as potential dye for food, textile, cosmetics, pharmaceutics and polyolefins (Danevčič et al, 2016; Davient et al, 2018; Dong et al, 2014; Genes et al, 2011; Goncharova et al, 2012). Prodigiosin is a critical bioactive compound with common applications in the food, pharmaceutical, cosmetic and textile industries. New strains are still being isolated from the surroundings, because different natural products extracted from different strains might have novel bioactivities (Auparakkitanon, 2014; Darshan & Manonmani, 2016; Dhar Purkayastha et al, 2018; Sturz et al, 1997; Suryawanshi et al, 2015)

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