Abstract
The Pantoea agglomerans 8488 lipopolysaccharide (LPS) was isolated, purified and characterized by monosaccharide and fatty acid analysis. The O-polysaccharide and lipid A components of the LPS were separated by mild acid degradation. Lipid A was studied by electrospray ionization mass spectrometry (ESI-MS) and found to consist of hexa-, penta-, tetra- and tri-acylated species. Two-dimensional nuclear magnetic resonance (NMR) spectroscopy revealed the following structure of the O-polysaccharide repeating unit →3)-α-L-Rhap-(1→6)-α-D-Manp-(1→3)-α-L-Fucp-(1→3)-β-D-GlcNAcp-(1→. The LPS showed a low level of toxicity, was not pyrogenic, and reduced the adhesiveness index of microorganisms to 2.12, which was twofold less than the control. LPS modified by complex compounds of germanium (IV) and tin (IV) were obtained. It was found that six LPS samples modified by Sn compounds and two LPS samples modified by Ge compounds lost their toxic activity when administered to mice in a dose of LD50 (105 µg/mice or 5 mg/kg). However, none of the modified LPS samples changed their serological activity in an Ouchterlony double immunodiffusion test in agar.
Highlights
IntroductionPantoea agglomerans (formerly Enterobacter agglomerans, Erwinia herbicola) is a ubiquitous bacterial strain that belongs to the Enterobacteriaceae family [1]
Pantoea agglomerans is a ubiquitous bacterial strain that belongs to the Enterobacteriaceae family [1]
The LPS was isolated from P. agglomerans 8488 cells by the phenol-water method with a yield of 10.9%
Summary
Pantoea agglomerans (formerly Enterobacter agglomerans, Erwinia herbicola) is a ubiquitous bacterial strain that belongs to the Enterobacteriaceae family [1]. It is primarily an environmental and agricultural organism that inhabits plants, soil, water, air and dust. P. agglomerans strains are frequently found in humans and isolated in hospitals. They might affect various organs in the body, ranging from non-life-threatening skin infections to fatal multiorgan systemic disorders [2,3]. P. agglomerans strains can have a positive effect by stimulating the production of substances effective for the treatment of cancer and other diseases [3,4]. LPS is known as a potent immunostimulator in both humans and animals
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