Structure of Muramidase (Lysozynie)

Abstract

In order to obtain information on the factors which stabilize the internal fold of the muramidase molecule, the effects of methanol, isopropanol, n-propanol, glycerol, α monochlorohydrin and ethylene glycol monomethyl ether on the optical rotatory and ultraviolet spectral properties have been studied. The following observations have been made: The effectiveness to disrupt the internal fold of the muramidase molecule increases with both chain length and increasing the hydrocarbon content.On the other hand, increasing the hydroxyl content of the solvent molecule had no significant effect on the internal fold. Thus, ethylene glycol and glycerol disrupt neither the internal fold nor the helical part.These observations suggest that the internal fold of the muramidase molecule is stabilized by hydrophobic forces.While there is no evidence for an increase in the helical Content of the muramidase molecule in the presence of methanol or ethanol, isopropanol and n-propanol increase the helical content.An increase in the helical content of the muramidase molecule was observed it acidic aqueous solutions of methanol, ethanol or isopropanol. The optical rotatory and ultraviolet spectral properties in these solvent are quite similar to those in aqueous 2-chioro ethanol in which hydrochloric acid is present α-Monochlorohydrin which contains hydro chloric acid also increases the helical content Thus, the acidic aqueous solutions of alcohol and the related compounds also can act a helix-former for muramidase. The effectiveness to disrupt the internal fold of the muramidase molecule increases with both chain length and increasing the hydrocarbon content. On the other hand, increasing the hydroxyl content of the solvent molecule had no significant effect on the internal fold. Thus, ethylene glycol and glycerol disrupt neither the internal fold nor the helical part. These observations suggest that the internal fold of the muramidase molecule is stabilized by hydrophobic forces. While there is no evidence for an increase in the helical Content of the muramidase molecule in the presence of methanol or ethanol, isopropanol and n-propanol increase the helical content. An increase in the helical content of the muramidase molecule was observed it acidic aqueous solutions of methanol, ethanol or isopropanol. The optical rotatory and ultraviolet spectral properties in these solvent are quite similar to those in aqueous 2-chioro ethanol in which hydrochloric acid is present α-Monochlorohydrin which contains hydro chloric acid also increases the helical content Thus, the acidic aqueous solutions of alcohol and the related compounds also can act a helix-former for muramidase.